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and Photoluminescence for Condensed Matter Studies
| Leaders: | G.M. Arzumanyan N. Kučerka |
Participating countries and international organizations:
Armenia, Belarus, Bulgaria, Poland, Romania, Russia, Serbia, Slovakia, Uzbekistan.
Issues addressed and main goals of research:
Fundamental and applied research in the field of spontaneous and nonlinear Raman microspectroscopy aimed at highly sensitive biosensing. Study of mechanisms and nature of surface-enhanced Raman scattering (SERS) taking into account the anomalous ratio of line inteities in the anti-Stokes and Stokes spectral regions. Applied research is aimed at the use of Raman spectroscopy and fluorescence microscopy in biomedical research, in particular, in the search for spectral markers of NETosis, as well as some features of lipid-protein interactions.
Expected results in the current year:
- Detection of Raman spectra of organic molecules in the low-frequency region using Bragg filters simultaneously in the Stokes and anti-Stokes regions.
- Investigation of a possible anomaly in the ratio of the peaks aSt / St in the SERS spectra depending on the continuous and pulsed pumping modes.
- Synthesis of liposomes / liposomes with embedded proteins and the study of their chemical structure and morphology by Raman spectroscopy and fluorescence microscopy.
- UV-induced NETOSIS: identification of dependence on wavelength and intensity of irradiation.
- Application of fluorescence microscopy to identify programmed death of neutrophil cells under the influence of various activators, including UV radiation.
| List of projects: | |||
| Project | Leaders | Priority (period of realisation) |
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| 1. | BIOPHOTONICS | G.M. Arzumanyan N. Kučerka Deputy: K.Z. Mamatkulov |
1 (2021-2023) |
| List of Activities | |||||
| Activity or Experiment | Leaders | ||||
| Laboratory or other Division of JINR |
Main researchers | ||||
| 1. | Study of the features of Stokes and anti-Stokes components of SERS spectra from analyte molecules in order to understand the processes of enhancement in SERS spectroscopy | G.M. Arzumanyan |
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| FLNP | Ye. Arynbek, K.Z. Mamatkulov, I.A. Morkovnikov, 2 engineers | ||||
| 2. | Determination of the range of pump intensities for recording reproducible aSt / St spectra | G.M. Arzumanyan K.Z. Mamatkulov |
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| FLNP | Ye. Arynbek, M.Yu. Vorobyeva, 1 engineer | ||||
| 3. | Stabilization of membrane proteins and studies of their structure using lipodiscs/liposomes by Raman spectroscopy, electron microscopy and SANS | G.M. Arzumanyan N. Kučerka |
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| FLNP | A. Damir, K.Z. Mamatkulov, M.Yu. Vorobyeva | ||||
| 4. | Testing the technique for obtaining Raman spectra of lipodiscs/liposomes with membrane proteins and “empty” lipodiscs/liposomes | K.Z. Mamatkulov N. Kučerka |
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| FLNP | Ye. Arynbek, A. Damir, M.Yu. Vorobyeva, 1 engineers | ||||
| 5. | Study of the influence of lipid environment on the structure of membrane protein | G.M. Arzumanyan N. Kučerka |
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| FLNP | K.Z. Mamatkulov, M.Yu. Vorobyeva, 1 engineer, 2 laboratory assistant | ||||
| 6. | Search for spectral / Raman markers of NETosis | G.M. Arzumanyan N. Kučerka |
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| FLNP | K.Z. Mamatkulov, M.Yu. Vorobyeva, 1 engineer, 2 laboratory assistant | ||||
| 7. | Study of mechanisms of sterile activation of NETosis under UV radiation | G.M. Arzumanyan K.Z. Mamatkulov |
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| FLNP | A. Damir, M.Yu. Vorobyeva, D.S. Zakritnaya | ||||
| 8. | Mastering the technique of ultra-low frequency Raman spectroscopy ~ (5-10) cm-1 | G.M. Arzumanyan K.Z. Mamatkulov |
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| FLNP | Ye. Arynbek, M.Yu. Vorobyeva, 2 engineers | ||||
Collaboration
| Country or International Organization | City | Institute or laboratory |
| Armenia | Yerevan | Foundation ANSL |
| Belarus | Minsk | BSUIR |
| SOL instruments | ||
| Bulgaria | Sofia | ISSP BAS |
| Poland | Krakow | JU |
| Romania | Brasov | UNITBV |
| Bucharest | CSSNT-UPB | |
| Cluj-Napoca | INCDTIM | |
| Magurele | NIMP | |
| Russia | Moscow | GPI RAS |
| LMPR MONIKI | ||
| Serbia | Belgrade | Univ. |
| Slovakia | Kosice | UPJS |
| Uzbekistan | Jizzakh | JSPI |
