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and Photoluminescence for Condensed Matter Studies
| Leaders: | G.M. Arzumanyan N. Kučerka |
Participating countries and international organizations:
Armenia, Belarus, India, Kazakhstan, Poland, Romania, Russia, Serbia, Slovakia, Uzbekistan.
Issues addressed and main goals of research:
Fundamental and applied research in the field of spontaneous and nonlinear Raman microspectroscopy aimed at highly sensitive biosensing. Study of mechanisms and nature of surface-enhanced Raman scattering (SERS) taking into account the anomalous ratio of line intensities in the anti-Stokes and Stokes spectral regions. Applied research is aimed at using Raman spectroscopy and fluorescence microscopy in biomedical research, in particular, in the search for spectral markers of NETosis, as well as some features of lipid-protein interactions.
Expected results in the current year:
- Investigation of a possible anomaly in the ratio of aSt/St peaks in SERS spectra depending on the pump power.
- Elucidation of conformational changes in the Raman spectrum of proteins in the presence of membrane mimetics.
- Light-induced NETosis upon excitation of neutrophil cells by radiation in the visible region of the spectrum: identification of mechanisms and signaling pathways.
- Application of fluorescence microscopy to identify programmed death of neutrophil cells under the influence of various activators, including UV- and visible radiation.
| List of projects: | |||
| Project | Leaders | Priority (period of realisation) |
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| 1. | BIOPHOTONICS | G.M. Arzumanyan N. Kučerka Deputy: K.Z. Mamatkulov |
1 (2021-2023) |
| List of Activities | |||||
| Activity or Experiment | Leaders | ||||
| Laboratory or other Division of JINR |
Main researchers | ||||
| 1. | Study of the features of Stokes and anti-Stokes components of SERS spectra from analyte molecules in order to understand the processes of enhancement in SERS spectroscopy | G.M. Arzumanyan |
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FLNP |
K.Z. Mamatkulov, Ye. Arynbek, I.A. Morkovnikov, 2 engineers |
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| 2. | Determination of the range of pump intensities for recording reproducible aSt/St spectra | G.M. Arzumanyan K.Z. Mamatkulov |
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FLNP |
Ye. Arynbek, A.A. Shutikov, 1 engineer |
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| 3. | Stabilization of membrane proteins and studies of their structure using liposomes / lipodiscs by Raman spectroscopy, electron microscopy and SANS | G.M. Arzumanyan N. Kučerka |
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FLNP |
K.Z. Mamatkulov, A. Damir, A.A. Shutikov, |
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| 4. | Testing the technique for obtaining Raman spectra of liposomes / lipodiscs with membrane proteins and “empty” liposomes / lipodiscs | K.Z. Mamatkulov N. Kučerka |
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FLNP |
Ye. Arynbek, A. Damir, A.A. Shutikov, |
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| 5. | Study of the influence of lipid environment on the structure of membrane protein | G.M. Arzumanyan N. Kučerka |
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FLNP |
K.Z. Mamatkulov, Ye. Arynbek, 1 engineer, |
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| 6. | Search for spectral / Raman markers of NETosis | G.M. Arzumanyan K.Z. Mamatkulov |
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FLNP |
Ye Arynbek, E.M. Demina, I.M. Pugachevskaya, |
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| 7. | Study of mechanisms of sterile activation of NETosis under UV- and visible radiation | G.M. Arzumanyan K.Z. Mamatkulov |
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FLNP |
Ye. Arynbek, E.M. Demina, D.S. Kravtsunova, |
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| 8. | Mastering the technique of ultra-low frequency Raman spectroscopy ~ 10 cm-1 | G.M. Arzumanyan K.Z. Mamatkulov |
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| FLNP | Ye. Arynbek, A.A. Shutikov, 2 engineers | ||||
Collaboration
| Country or International Organization | City | Institute or Laboratory |
| Armenia | Yerevan | Foundation ANSL |
| Belarus | Minsk | BSUIR |
| SOL instruments | ||
| India | Aizawl | MZU |
| Kazakhstan | Almaty | INP |
| Poland | Krakow | JU |
| Romania | Cluj-Napoca | UBB |
| Magurele | NIMP | |
| Russia | Moscow | GPI RAS |
| MSU | ||
| Serbia | Belgrade | Univ. |
| Slovakia | Kosice | UPJS |
| Uzbekistan | Jizzakh | JSPI |
